A simple high performance thin layer chromatography method is developed. The determination was carried on silica gel 60 gf254 hptlc plates using a mobile phase of Toluene : Ethyl Acetate : Methanol : Acetic acid (6:3:1:0.1 v/v/v/v). The absorbance of the spots was measured by densitometry at 289 nm. The retention Factor (Rf) For omeprazolewas 0.75,and for cinitapride hydrogen tartrate 0.48. Omeprazole and Cinitapride hydrogen tartrate showed a linear response in the concentrarion range 100-600ng and 15-90ng/band respectively. The correlation co-efficient (R2 value) for Omeprazole and Cinitapride hydrogen tartrate was 0.996 and 0.995 for both drug. The result of analysis have been validated statistically and by recovery studies. The percentage recoveries obtained for Omeprazole and Cinitapride hydrogen tartrate ranges from 97.86 To 99.20.
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